Transcriptomics is the study of the entirety of an organism's RNA transcripts. Transcriptomics allows us to understand gene expression levels in different tissues, conditions or timepoints [1]. This may be evaluated using a variety of techniques including DNA microarrays and RNA-sequencing followed by expression profile clustering.
Results
RNA expression levels of FOXN1 transcripts ins different tissues
RNA expression clustering with annotation identifies FOXN1 as part of the skin cluster in this UMAP. The 15 genes on the right are the 10 with the most similar expression profiles.
Discussion
FOXN1 was shown to be highly expressed in the skin, but also in lymphoid tissue, the proximal digestive tract and female tissues. Additionally, this transcript was recognized as part of skin-cornification cluster. Cornification is the process involving epidermal cell differentiation and programmed cell death that creates the outermost layer of our skin as well as hair and nail follicles.
Sources
1. Lowe, R., Shirley, N., Bleackley, M., Dolan, S., & Shafee, T. (2017). Transcriptomics technologies. PLoS computational biology, 13(5), e1005457. https://doi.org/10.1371/journal.pcbi.1005457
IMAGES: Biorender
This web page was produced as an assignment for Genetics 564, a capstone course at UW-Madison.